> For the complete documentation index, see [llms.txt](https://tbj128.gitbook.io/mian/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://tbj128.gitbook.io/mian/rarefaction-curves.md).

# Rarefaction Curves

Draws a series of rarefaction curves on the OTU table prior to subsampling. This analysis relies on the rarecurve function in the R 'vegan' package [here](https://www.rdocumentation.org/packages/vegan/versions/2.4-2/topics/rarefy)

![](/files/-M39x94S1kzfFeWbJTYm)

### Used For

* Draws a series of rarefaction curves on the OTU table prior to subsampling

{% hint style="info" %}
This tool works best when the original uploaded OTU table was not subsampled
{% endhint %}

### Visualization Parameters

#### Color Variable

Create comparative sample groups based on categorical variables uploaded in the metadata file.

Optionally create a categorical variable from a quantitative variable by using the Quantile Range feature on the Projects home page.

Each sample group is assigned its own color.

### Interactive Elements

* Hover over each point to determine the sample ID, corresponding sample group, and number of OTUs at a particular sample size

### Additional Features

* **Save Snapshot**: Save the visualization to the experiment notebook
* **Download**: Downloads the visualization as a PNG file
* **Share**: Creates a shareable link that allows you to share the visualization with others


---

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