# Rarefaction Curves

Draws a series of rarefaction curves on the OTU table prior to subsampling. This analysis relies on the rarecurve function in the R 'vegan' package [here](https://www.rdocumentation.org/packages/vegan/versions/2.4-2/topics/rarefy)

![](https://2045057293-files.gitbook.io/~/files/v0/b/gitbook-legacy-files/o/assets%2F-M32ms-dWLPjFImgSINF%2F-M39x10gFa-9w4PZQYWW%2F-M39x94S1kzfFeWbJTYm%2Fimage.png?alt=media\&token=05e8b0e9-2a3d-460c-887f-4d1aaed448b3)

### Used For

* Draws a series of rarefaction curves on the OTU table prior to subsampling

{% hint style="info" %}
This tool works best when the original uploaded OTU table was not subsampled
{% endhint %}

### Visualization Parameters

#### Color Variable

Create comparative sample groups based on categorical variables uploaded in the metadata file. 

Optionally create a categorical variable from a quantitative variable by using the Quantile Range feature on the Projects home page. 

Each sample group is assigned its own color.

### Interactive Elements

* Hover over each point to determine the sample ID, corresponding sample group, and number of OTUs at a particular sample size

### Additional Features

* **Save Snapshot**: Save the visualization to the experiment notebook
* **Download**: Downloads the visualization as a PNG file
* **Share**: Creates a shareable link that allows you to share the visualization with others