# Rarefaction Curves

Draws a series of rarefaction curves on the OTU table prior to subsampling. This analysis relies on the rarecurve function in the R 'vegan' package [here](https://www.rdocumentation.org/packages/vegan/versions/2.4-2/topics/rarefy)

![](/files/-M39x94S1kzfFeWbJTYm)

### Used For

* Draws a series of rarefaction curves on the OTU table prior to subsampling

{% hint style="info" %}
This tool works best when the original uploaded OTU table was not subsampled
{% endhint %}

### Visualization Parameters

#### Color Variable

Create comparative sample groups based on categorical variables uploaded in the metadata file.

Optionally create a categorical variable from a quantitative variable by using the Quantile Range feature on the Projects home page.

Each sample group is assigned its own color.

### Interactive Elements

* Hover over each point to determine the sample ID, corresponding sample group, and number of OTUs at a particular sample size

### Additional Features

* **Save Snapshot**: Save the visualization to the experiment notebook
* **Download**: Downloads the visualization as a PNG file
* **Share**: Creates a shareable link that allows you to share the visualization with others


---

# Agent Instructions: Querying This Documentation

If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter:

```
GET https://tbj128.gitbook.io/mian/rarefaction-curves.md?ask=<question>
```

The question should be specific, self-contained, and written in natural language.
The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.